Effects of Hyperglycemia on Vascular Smooth Muscle Ca²⁺ Signaling

Figure 2

Effect of glucose normalization on Ca²⁺ release in A7r5 VSM cells. PE induced Ca²⁺ release (a, b) and IP3R expression (c) in response to different glycemic conditions. Cells cultured under HG, NG, or OC were loaded with Fura-2AM and Ca²⁺ transients imaged following exposure to different concentrations of PE (1–20 μM) (a, b). IP3R expression from cells cultured under HG, NG, and OC was determined by western blot analysis and quantified, as described in Materials and Methods (c). Data are presented as mean ± SE from at least three independent experiments done each in triplicate.

(a)

(b)

(c)