Effects of Hyperglycemia on Vascular Smooth Muscle Ca²⁺ Signaling

Figure 4

Effect of glucose on voltage gated channels (VGCC) in A7r5 VSM cells. Ca²⁺ transients (a, b) and VSM contraction (c, d) in response to a 20 mM KCl depolarizing pulse to activate VGCC in A7r5 cells. Cytosolic Ca²⁺ transients in Fura2-AM loaded A7r5 cells, cultured under HG, NG, and OC in response to 20 mM KCl (a, b). Effect of glucose normalization on A7r5 cells contractility in response to 20 mM KCl was determined as described in Materials and Methods. Max contraction is presented after normalization to basal level (c, d). Data are presented as from at least three independent experiments done each in triplicate. ; NS: not significant.

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