Loss of PI3K p110α in the Adipose Tissue Results in Infertility and Delayed Puberty Onset in Male Mice

Table 1

Primer sequences for the genes tested and references.

Gene	Forward primer	Reverse primer	Reference
StAR; (Star)	ACAACCAGGAAGGCTGGAAG	ATGCAGGTGGGGCCGTGTTCA	[23]
P450scc protein; (Cyp11a1)	AGGTCCTTCAATGAGATCCCTT	TCCCTGTAAATGGGGCCATAC	[23]
Aromatase; (Cyp19a1)	TGTGTTGACCCTCATGAGACA	CTTGACGGATCGTTCATACTTC	[24]
17α-Hydroxylase; (Cyp17a1)	GGAGAGTTTGCCATCCCGAA	CGCTCAGGCATAAACCGAT	[25]
17-beta-hydroxysteroid dehydrogenase; (Hsd17b3)	ATTTTACCAGAGAAGACATCT	GGGGTCAGCACCTGAATAATG	[23]
Leptin; (Lep)	TGACACCAAAACCCTCATCA	AGCCCAGGAATGAAGTCCA	[26]
TNF-α; (Tnf)	CCACCACGCTCTTCTGTCTA	AGCTGCTCCTCCACTTGGTG	[26]
LepR; (Lepr)	AGAACGGACACTCTTTGAAGTCT	AACCATAGTTTAGGTTTGTTTC	[27]
IR; (Insr)	GAGAGGATGTGAGACGACG	CAGGTTGTTCCGGATGTCC	[26]
AR; (Ar)	TGCCCGAATGCAAAGGTCTT	TTGGCGTAACCTCCCTTGAAA	[28]
ERα; (Esr1)	GGTGCCCTACTACCTGGAG	GCCCACTTCGTAACACTTGCGC	[29]
β-Actin; (Actb)	AGGCCCAGAGCAAGAGAG	GGGTGTTGAAGGTCTCAAAC	[26]

StAR, steroidogenic acute regulatory enzyme, LepR, leptin receptor, IR, insulin receptor, AR, androgen receptor, ERα, estrogen receptor alpha, TNF-α, tumor necrosis factor. In parenthesis is the gene encoding the protein.