Knockdown of SIRT1 Suppresses Bladder Cancer Cell Proliferation and Migration and Induces Cell Cycle Arrest and Antioxidant Response through FOXO3a-Mediated Pathways

Table 4

List of secondary antibodies and counterstaining of nuclei.

Secondary detection system used	Host	Method	Dilution	Supplier
Anti-mouse-IgG (H + L)-HRP	Goat	WB	1 : 10,000	Sungene Biotech, China, Cat. #LK2003
Anti-rabbit-IgG (H + L)-HRP	Goat	WB	1 : 10,000	Sungene Biotech, China, Cat. #LK2001
Anti-rabbit IgG (H + L), F(a)2 fragment (Alexa Fluor® 488 Conjugate)	Goat	IF	1 : 50	Cell Signaling Technology, USA, Cat. #4412
Anti-mouse IgG (H + L), F(a)2 fragment (Alexa Fluor® 555 Conjugate)	Goat	IF	1 : 50	Cell Signaling Technology, USA, Cat. #4408
Anti-goat IgG-FITC	Rabbit	IF	1 : 100	Boster Biological Technology, China, Cat. #BA1110
Anti-goat IgG-Cy3	Rabbit	IF	1 : 100	Boster Biological Technology, China, Cat. #BA1034
Hoechst 33342 nucleic acid staining (DAPI)	—	IF	1 : 750	Molecular Probes/Invitrogen, USA, Cat. #A11007