BioMed Research International / 2017 / Article / Tab 4

Research Article

Knockdown of SIRT1 Suppresses Bladder Cancer Cell Proliferation and Migration and Induces Cell Cycle Arrest and Antioxidant Response through FOXO3a-Mediated Pathways

Table 4

List of secondary antibodies and counterstaining of nuclei.

Secondary detection system usedHostMethodDilutionSupplier

Anti-mouse-IgG (H + L)-HRPGoatWB1 : 10,000Sungene Biotech, China, Cat. #LK2003
Anti-rabbit-IgG (H + L)-HRPGoatWB1 : 10,000Sungene Biotech, China, Cat. #LK2001
Anti-rabbit IgG (H + L), F(a)2 fragment (Alexa Fluor® 488 Conjugate)GoatIF1 : 50Cell Signaling Technology, USA, Cat. #4412
Anti-mouse IgG (H + L), F(a)2 fragment (Alexa Fluor® 555 Conjugate)GoatIF1 : 50Cell Signaling Technology, USA, Cat. #4408
Anti-goat IgG-FITCRabbitIF1 : 100Boster Biological Technology, China, Cat. #BA1110
Anti-goat IgG-Cy3RabbitIF1 : 100Boster Biological Technology, China, Cat. #BA1034
Hoechst 33342 nucleic acid staining (DAPI)IF1 : 750Molecular Probes/Invitrogen, USA, Cat. #A11007

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