Hypothyroidism Reduces the Size of Ovarian Follicles and Promotes Hypertrophy of Periovarian Fat with Infiltration of Macrophages in Adult Rabbits

Figure 3

Expression of the cytochrome P450 aromatase in the ovary of control (C; open bar; ) and hypothyroid (Hypo; solid bar; ) rabbits. Representative immunoblot showing the expression of aromatase (a) and Ponceau’s Red stained membrane (b). Relative expression of aromatase in C and Hypo groups (c). Data are mean ± SEM. . Immunoreactivity of antiaromatase (+) was identified in granulosa cells of antral follicles (tertiary, tf; Graafian, gf) of control (d, e) and hypothyroid (f, g) ovaries. Nonlabeling was observed when the primary antibody was omitted (negative control; h). Bars: (d) 200 μm; (f) and (h) 100 μm; and (e) and (g) 20 μm. Granulose cells, gc; theca cells, tc.

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