Chlamydial Type III Secretion System Needle Protein Induces Protective Immunity against Chlamydia muridarum Intravaginal Infection

Figure 4

Antibodies against Chlamydia muridarum TC_0037 neutralize chlamydia infectivity in vitro. C. muridarum elementary bodies were opsonized with sera from immunized or control mice () at different dilutions and used to infect McCoy cell monolayers. Inclusions were counted 48 h after infection. Neutralization activity was determined by measuring the reduction in the number of inclusions generated by antibody-opsonized elementary bodies. The graphs present the percent neutralization as an average of experiments performed in triplicate with standard deviations ().