(a)
(b)
(c)
(d)
Figure 4: ROS production was blocked in T. vaginalis-treated SiHa cells after pretreatment with N-acetyl-L-cysteine (NAC). (a and b) Intracellular ROS production was detected by dihydroethidium (DHE) fluorescence. Intracellular ROS levels were concentration-dependently inhibited in T. vaginalis-treated SiHa cells following NAC pretreatment. (c and d) Mitochondrial ROS production was detected by MitoSOX reagent. Mitochondrial ROS production was concentration-dependently prevented in T. vaginalis-treated SiHa cells following NAC pretreatment. The experiment was repeated three times with similar results. Scale bars: 100 μm; versus SiHa cell control, versus SiHa cell control.