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BioMed Research International
Volume 2017, Article ID 3921258, 9 pages
https://doi.org/10.1155/2017/3921258
Research Article

Expression Levels and Clinical Significance of miR-21-5p, miR-let-7a, and miR-34c-5p in Laryngeal Squamous Cell Carcinoma

1Department of Otorhinolaryngology, Umberto I University General Hospital, Università Politecnica delle Marche, Via Conca 71, 60126 Ancona, Italy
2Department of Otorhinolaryngology, Audiology and Phoniatrics “G. Ferreri”, University La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
3Department of Molecular and Clinical Sciences, Università Politecnica delle Marche, Via Tronto 10/a, 60126 Ancona, Italy
4Pathologic Anatomy and Histopathology Division, Department of Biomedical Sciences and Public Health, Università Politecnica delle Marche, Via Conca 71, 60126 Ancona, Italy
5Department of Biomedical Sciences and Public Health, Section of Medical Statistics, Faculty of Medicine, Università Politecnica delle Marche, Via Tronto 10/a, 60126 Ancona, Italy
6Center of Clinical Pathology and Innovative Therapy, INRCA-IRCCS Italian National Institute, Via della Montagnola 81, 60127 Ancona, Italy

Correspondence should be addressed to Arisa Bajraktari; ti.oohay@38iratkarjab

Received 26 February 2017; Accepted 1 August 2017; Published 31 August 2017

Academic Editor: Vickram Ramkumar

Copyright © 2017 Massimo Re et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Altered microRNAs (miRNAs) expression has been found in many cancer types, including laryngeal squamous cell carcinoma (LSCC). The aim of this study was to determine the role and clinical value of three LSCC-related miRs, such as miR-21-5p, miR-let-7a, and miR-34c-5p in a homogeneous cohort of patients with primary LSCC treated by primary surgery. Methods. Expression levels of miR-21-5p, miR-let-7a, and miR-34c-5p were detected in 43 pairs of LSCC and adjacent normal tissues by reverse-transcription quantitative PCR. Overall survival and disease-free survival were evaluated using the Kaplan–Meier method, and multivariate analysis was performed using the Cox proportional hazard analysis. Results. miR-21-5p is significantly upregulated, while miR-let-7a is significantly downregulated in LSCC tumor tissues compared with the corresponding adjacent normal tissues. The downregulation of miR-34c-5p expression significantly correlated with a shorter disease-free survival and, in the multivariate analysis, low miR-34c-5p expression was associated with an increased risk of recurrence. Conclusions. miR-21-5p, miR-let-7a, and miR-34c-5p seem to play a critical role in LSCC carcinogenesis and might have a diagnostic and prognostic clinical value. The miR-let-7a levels could have a predictive role for lymph node metastases and miR-34c-5p might be a promising biomarker of patient outcome.