The Use of Xanthan Gum as Vaccine Adjuvant: An Evaluation of Immunostimulatory Potential in BALB/c Mice and Cytotoxicity In Vitro

Figure 5

Quantification of IFN-γ in splenocytes culture by ELISA. Spleen cells were isolated and cultured in 24-well tissue culture plates (5 × 10⁶ cells/well) for 48 h. The levels of the IFN-γ in cell-free culture supernatants were measured by ELISA. The values represent the mean ± SD of the three independents experiments. For statistical analysis, concentration values for the immunized groups treated with the OVA were compared with those for the OVA-saline immunized group treated with OVA, and bars with different letters represent .