Connexin43 and AMPK Have Essential Role in Resistance to Oxidative Stress Induced Necrosis

Figure 2

siRNA knockdown or blocking Cx43 by Cbx enhanced H₂O₂-induced necrosis in astrocytes. (a) Presentative bands expression of Cx43 was detected by western blotting. (b) Quantification of Cx43/GAPDH by membrane intensity. (c) Necrosis and apoptosis cells were stained by Annexin V FITC and PI and determined by flow cytometry (n = 10,000 cells). indicated ; indicated .

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