Overexpression of CaAPX Induces Orchestrated Reactive Oxygen Scavenging and Enhances Cold and Heat Tolerances in Tobacco

Figure 1

Sequence analysis and gene expression profiles of CaAPX. (a) Phylogenetic tree analysis of CaAPX and related proteins. (b) Alignment of amino acid sequences. Ca, C. azalea (KP635267); At, Arabidopsis thaliana (NP_172267); Gh, Gossypium hirsutum (ABR18607); Gm, Glycine max (NP_001237785); Nt, Nicotiana tomentosiformis (XP_009597491); Os, Oryza sativa (japonica cultivar group) (P93404); Pd, Phoenix dactylifera (XP_008783664); St, Solanum tuberosum (NP_001275066). Red boxes indicate the highly conserved APX active-site signature. (c) Relative expression of CaAPX in different tissues of C. azalea. (d) Relative expression of CaAPX in leaves of C. azalea during different times under 4°C cold stress. (e) Relative expression of CaAPX in leaves of C. azalea during different times under 38°C hot stress. The data are the means of four replicates and were compared by Duncan’s multiple range tests.

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