Overexpression of CaAPX Induces Orchestrated Reactive Oxygen Scavenging and Enhances Cold and Heat Tolerances in Tobacco

Figure 2

Analysis of transgenic plants. (a) Phenotypes of transgenic and WT plants under cold and hot stress treatments after 8 h. Upper panel, 4 degrees; bottom panel, 38 degrees. (b) Southern blot analysis of transgenic lines. (c) Detection of CaAPX transgenic tobacco by RT-PCR. Expression level of CaAPX gene in different lines (L1, L2, and L3) of transgenic and wild type (WT) plants. M2: λ-Hind III digested DNA marker; L1, L2, and L3: 3 lines of CaAPX transgenic tobacco; +: positive control of pBI121-CaAPX combined vector; −: water as the negative control; WT: wild type. The data are the means of four replicates and were compared by Duncan’s multiple range tests.

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