A Novel Cyclophilin B Gene in the Red Tide Dinoflagellate Cochlodinium polykrikoides: Molecular Characterizations and Transcriptional Responses to Environmental Stresses

Table 1

The primers used in the present study.

Primer name	Nucleotide sequence (5′→3′)	Remarks
5-SL	CCGTAGCCATTTTGGCTCAAG	5′-RACE
CpCyp5R1	TGTCCTTGCCAGCATTGG	5′-RACE
CpCyp5R2	CGTCTTGGGCACCTTCTT	5′-RACE
B25	GACTCTAGACGACATCGA	3′-RACE
B26	GACTCTAGACGACATCGA(T)18	3′-RACE
CpCypF1	CATGACGATGGTGTTGCTAAG	3′-RACE/genomic DNA/full length
CpCypF2	GTTGCTAAGGGGACTATTTGC	3′-RACE/full length
CpCyp3F3	AAGAAGGTGCCCAAGACG	Full length
CpCypR1	GTTGAGCGTGCCCTTGTA	Genomic DNA/full length
CpCypR0	GACAACTCACAGCTCCTCGT	Genomic DNA/full length
CpCypR3	CACATGGCAAATAGTCCCCT	Intergenic DNA
CpCypF3	AGCCGTACAAGGATGCCT	Intergenic DNA
CpCypR4	CTTAGCAACACCATCGTCATG	Intergenic DNA
CpCypF4	GCTTTCAACGAGGAGCTGT	Intergenic DNA
CpCypR5	TTGATGACGCGGTGGAAC	RT-PCR
CpCypF5	TCTACGGCAAGAAGGTCC	RT-PCR
Cp-TUA2-F2	TTCTCGCGCATCGACCACAAG	RT-PCR
Cp-TUA2-R2	TCCATACCCTCGCCGACATAC	RT-PCR