Salinomycin Exerts Anticancer Effects on PC-3 Cells and PC-3-Derived Cancer Stem Cells In Vitro and In Vivo

Figure 1

Salinomycin inhibits cell viability and induces apoptosis in PC-3 cells. (a) Cell viability was evaluated by MTT assays. The various concentrations of salinomycin were added to RWPE-1 and PC-3 cells for 12 h, 24 h, and 48 h. The IC₅₀ values of salinomycin in PC-3 and RWPE-1 cells for the indicated time periods. (b) Chromatin condensation of RWPE-1 and PC-3 cells was induced by salinomycin. Cells were stained with DAPI and viewed under a fluorescence microscope. Scale bars: 10 μm. (c) AO/EB staining of RWPE-1 and PC-3 cells, followed by viewing under a fluorescence microscope. Scale bars: 10 μm. (d) Salinomycin induced RWPE-1 and PC-3 cells apoptosis. The annexin V-FITC/PI staining shows the rate of apoptosis after salinomycin treatment. (e) The histogram shows the apoptosis rate (%) of cells. Data are presented as means ± SDs (). and versus control.

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