Salinomycin Exerts Anticancer Effects on PC-3 Cells and PC-3-Derived Cancer Stem Cells In Vitro and In Vivo

Figure 3

Salinomycin inhibits PC-3 cell proliferation and induces cell cycle arrest. (a) Salinomycin induced cell cycle arrest in PC-3 cells. The various concentrations of salinomycin were added to RWPE-1 and PC-3 cells for 24 h. Cell cycle progress was evaluated by flow cytometry. (b) The histogram shows the percentage of cells in G0/G1, S, and G2/M phases. (c) Salinomycin inhibits PC-3 cell proliferation. Salinomycin was added to RWPE-1 and PC-3 cells for 24 h. Cell proliferation was measured by colony formation assay. (d) The histogram shows the colony forming rate. Data are presented as means ± SDs (). versus control. (e and f) Changes in cell cycle regulatory proteins after salinomycin treatment for the indicated time periods and dose concentrations.

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