Salinomycin Exerts Anticancer Effects on PC-3 Cells and PC-3-Derived Cancer Stem Cells In Vitro and In Vivo

Figure 4

Salinomycin suppresses Wnt/β-catenin signaling pathway. (a and b) Salinomycin altered Wnt/β-catenin signaling pathway. PC-3 cells were treated with 5 μM salinomycin for 0, 2, 4, 6, and 8 h or treated with 0, 0.5, 1, 2, and 5 μM salinomycin for 8 h. The Wnt/β-catenin signaling pathway-related proteins were detected by western blot. (c) The immunofluorescence staining of β-catenin (red) in salinomycin-treated PC-3 cells; nuclei are stained with DAPI (blue). Scale bars: 10 μm.

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