Extrinsic Apoptosis Pathway Altered by Glycogen Synthase Kinase-3β Inhibitor Influences the Net Drug Effect on NSC-34 Motor Neuron-Like Cell Survival

Figure 3

Changes in the classical FADD-caspase-8 extrinsic apoptosis pathway markers after glycogen synthase kinase-3β (GSK-3β) inhibitor VIII treatment. Changes in immunoreactivity (IR) of the classical extrinsic apoptosis markers based on the treated GSK-3β inhibitor concentration are presented in an enhanced chemiluminescence radiograph as quantitative values. ((a) and (b)) IR of Fas and the Fas ligand, which are the first step in the FADD-caspase-8 extrinsic pathway, did not change in the different GSK-3β inhibitor-treated groups. (c) IR of cleaved caspase-8 increased in a dose-dependent manner. (d) IR of cytosolic cytochrome C, a common apoptosis marker, decreased in the low-dose group and was minimized at 200 nM. The 1000 nM GSK-3β inhibitor VIII-treated cells showed a U-shaped increasing IR pattern. (compared with control under serum deprivation only). (compared with 200 nM GSK-3β inhibitor-treated group).

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