Cervical Cancer Cell Line Secretome Highlights the Roles of Transforming Growth Factor-Beta-Induced Protein ig-h3, Peroxiredoxin-2, and NRF2 on Cervical Carcinogenesis

Figure 5

Schematic representation of NRF2 regulation and function, as predicted by IPA. (a) NRF2 or NFE2L2 (orange ellipse) action is predicted to be activated. All downstream genes are predicted to be upregulated and are colored in different shades of light pink to red, with darker color indicating higher degree of upregulation. The orange color indicates activation. (b) NRF2-mediated oxidative stress response is depicted. NRF2 binds to Keap1, following dependent (1), independent (2), or actin-dependent (3) mechanisms, described in detail in Discussion. NRF2 translocates to the nucleus, binds to antioxidant response elements (ARE/EpRE), and activates transcription of antioxidant and detoxifying enzymes, such as PRDX1 (peroxiredoxin-1), STIP1 (stress-induced-phosphoprotein-1), and VCP (transitional endoplasmic reticulum ATPase), which are upregulated (red color) according to the proteomics results.

(a)

(b)