Research Article

Effect of R119G Mutation on Human P5CR1 Dynamic Property and Enzymatic Activity

Figure 3

Purification analysis of R119G mutant by 12% SDS-PAGE. Marker, molecular mass markers. 1, before induction. 2, after induction. 3, mutant purified by Ni2+-chelating column. 4, mutant purified by SEC. 5, WT purified by SEC.