Effect of R119G Mutation on Human P5CR1 Dynamic Property and Enzymatic Activity

Table 2

Kinetic parameters for WT and mutant enzymes using 3,4-dehydro-L-proline as substrate and NAD⁺ as cofactor.

	(s^−1)	(mM)	(mM/min)	/ (s^−1M^−1)
	3,4-Dehydro-L-proline as the variable
WT	10.23	0.20	0.27	51150
Mutant	2.13	1.28	0.12	1664
	NAD^+ as the variable
WT	7.95	0.09	0.21	88333
Mutant	0.71	0.59	0.04	1203

was fixed at 1.0 mM.
was fixed at 352 M.