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BioMed Research International
Volume 2017 (2017), Article ID 4201809, 11 pages
https://doi.org/10.1155/2017/4201809
Research Article

Characterization of Exopolysaccharide Produced by Streptococcus thermophilus CC30

Department of Biotechnology, KL University, Green Fields, Vaddeswaram, Guntur 522502, India

Correspondence should be addressed to Sudhamani Muddada

Received 8 April 2017; Accepted 23 May 2017; Published 26 July 2017

Academic Editor: José D. Fontana

Copyright © 2017 Sri Lakshmi Ramya Krishna Kanamarlapudi and Sudhamani Muddada. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An exopolysaccharide (EPS) producing strain CC30 was isolated from raw milk and identified as Streptococcus thermophilus with morphological and 16S sequencing analysis. The strain was shown to produce 1.95 g/L of EPS when grown in skim milk lactose medium at 30°C by increasing the viscosity of the medium. The EPS was isolated and purified, and it was shown to consist of glucose and galactose in 1 : 1 ratio, with molecular weights ranging from 58 to 180 kDa. FTIR spectroscopy indicated the EPS to have amide, hydroxyl, and carboxyl groups. Under Atomic Force Microscopy, EPS showed spike-like lumps of EPS. Scanning Electron Microscopy (SEM) studies showed that it had irregular lumps with a coarse surface. The EPS displayed pseudoplastic nature. Thermogravimetric analysis (TGA) reported a degradation temperature of 110.84°C. The purified EPS exhibited reducing activity, hydrogen peroxide radical scavenging activity, and emulsification activity. The results of the present study indicated that EPS producing Streptococcus thermophilus could serve as a promising candidate for further exploitation in food industry.