Andrographolide Promotes Neural Differentiation of Rat Adipose Tissue-Derived Stromal Cells through Wnt/β-Catenin Signaling Pathway

Figure 1

Representative images of cell culture, staining results of differentiation, and flow cytometry analysis of CD marker expression in third-passaged ADSCs. (a) ADSCs showed a flattened fibroblast-like and fusiform morphology; cells in the third passage (p3) and fifth passage (p5) were arranged in a voxel shape. Scale bar: 200 μm. (b) Most ADSCs expressed CD90 (93.51%), CD44 (87.09%), and CD29 (95.65%) and relatively few expressed CD34 (1.19%), CD45 (0.96%), or CD11b, 2f, and c (1.15%). FITC-conjugated mouse IgG or FITC-conjugated hamster IgG was used as a negative control. (c) Adipogenesis (A) was demonstrated by oil red O staining, which revealed lipid droplets in the cytoplasm. Osteogenesis (B) was demonstrated by alizarin red S staining, which typically verified calcium deposits. Chondrogenesis (C) was demonstrated by alcian blue staining, which showed the acid mucopolysaccharide in chondrocyte balls. Scale bar: 200 μm.

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