Andrographolide Promotes Neural Differentiation of Rat Adipose Tissue-Derived Stromal Cells through Wnt/β-Catenin Signaling Pathway

Figure 3

Expression of neural markers proteins at 1 week after treatment. (a) Immunofluorescence staining demonstrated the expression of the NSC marker nestin in ADSCs, both before and after neural induction. Scale bar: 50 μm. (b) Immunofluorescence staining showed positive expression of the neuronal markers MAP2, tau, and β-tubulin III in the control and ANDRO groups, but weakly positive expression of the astrocytic marker GFAP. A semiquantitative analysis indicated there is no statistical difference between the two groups. Scale bar: 50 μm. (c) After exposure to an ANDRO-containing medium (2, 5, and 10 μM), Western blot analysis revealed a significant increase in the protein expression levels of MAP2, tau, β-tubulin III, and GFAP dose-dependently in the ANDRO group. < 0.001 compared with the control group.

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