A Novel Pan-Flavivirus Detection and Identification Assay Based on RT-qPCR and Microarray

Figure 2

Melting curves from different flaviviruses. Melting curve analysis of the amplicons from different flaviviruses (samples from the ANSES WNV Proficiency Test, 2013). NY99, It08, and Is985 are WNV lineage 1a strains, while Aus08 is a lineage 2 strain. The viruses were identified using the microarray, with confirmation by DNA sequencing.