Research Article
A Novel Pan-Flavivirus Detection and Identification Assay Based on RT-qPCR and Microarray
Figure 3
Hybridization patterns for different dilutions of viral samples. Cell culture supernatants: (a) West Nile virus lineage 1a, strain NY99 (WNV.1a.NY); red bars: sample diluted 10−1 and blue bars: diluted 10−4; (b) West Nile virus lineage 2, strain Uganda 1937 (WNV.2.Ug); red bars: 10−1 and blue bars: 10−4; (c) Murray Valley encephalitis virus (MVEV); red bars: 10−1 and blue bars: 10−3; (d) Tick-borne encephalitis virus, strain Langaat (TBLang); red bars: 10−1 and blue bars: 10−3. These data show that hybridization patterns are very sensitive to variation in the viral sequence, while remaining stable in a broad concentration range of viral RNA.
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