A Novel Pan-Flavivirus Detection and Identification Assay Based on RT-qPCR and Microarray

Table 1

Thermal cycling profiles used in the Flavivirus RT-qPCR.

Standard (4.5 h, 25 μL)	Fast (2.5 h, 25 μL or 10 μL)
(1) 50°C for 30 min; (2) 95°C for 15 min; (3) 95°C for 15 s; (4) 55°C for 25 s + plate read; (5) 72°C for 25 s + plate read; (6) 80°C for 1 s + plate read; (7) GOTO (3); 44 more times; (8) 95°C for 1 min; (9) Melting curve, 68 to 88°C,; increment 0.1°C, 1 s + plate read; (10) 4°C forever (optional); End	(1) 50°C for 30 min; (2) 95°C for 15 min; (3) 95°C for 15 s; (4) 55°C for 20 s; (5) 72°C for 20 s + plate read; (6) GOTO (3); 44 more times; (7) 95°C for 1 min; (8) Melting curve, 68 to 86°C,; increment 0.2°C, 1 s + plate read; End