Investigate the Effect of Thawing Process on the Self-Assembly of Silk Protein for Tissue Applications

Figure 1

Illustration of the thawing process: (a) IT designed for immediate thawing in 1 hour and (b) ST to control and decelerate the thawing process into 24 (ST24) and 48 (ST48) hours using water as a temperature regulator. While the IT process was the same for −20°C and −80°C frozen samples, the −80°C samples (b2) in ST process would be moved to and stocked at −20°C for 24 hours before being increased to 2°C and 8°C to be thawed (b1).