Inhibitory Activities of Stauntonia hexaphylla Leaf Constituents on Rat Lens Aldose Reductase and Formation of Advanced Glycation End Products and Antioxidant

Table 1

Inhibitory effect of crude extract and fractions of Stauntonia hexaphylla leaves on rat lens aldose reductase (RLAR), advanced glycation end products (AGEs), and antioxidant (DPPH).

Extract and fractions		IC50
		RLAR	AGEs	DPPH
Extract	70% EtOH
Fractions	Hex			>2000
	CH2Cl2
	EtOAc
	n-BuOH
	Water	>50	>500
Positive controls			—	—
		—		—
	L-ascorbic	—	—

IC₅₀ value was defined as a mean ± SEM of half-maximal inhibitory concentration from three independent experiments performed in duplicate.
is the positive control for aldose reductase.
is the positive control for advanced glycation end products.
-ascorbic acid is the positive control for DPPH.