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BioMed Research International
Volume 2017, Article ID 4276928, 14 pages
https://doi.org/10.1155/2017/4276928
Research Article

Culture of iPSCs Derived Pancreatic β-Like Cells In Vitro Using Decellularized Pancreatic Scaffolds: A Preliminary Trial

1Department of General Surgery, Affiliated Hospital of Nantong University, Nan Tong, Jiang Su, China
2Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nan Tong, Jiang Su, China
3Department of General Surgery, Affiliated Cancer Hospital of Nantong University, Nan Tong, Jiang Su, China
4Department of Emergency Surgery, Affiliated Hospital of Nantong University, Nan Tong, Jiang Su, China

Correspondence should be addressed to Yuhua Lu; moc.621@67hyl and Zhiwei Wang; moc.361@9363wzw

Received 31 August 2016; Revised 30 December 2016; Accepted 19 January 2017; Published 5 April 2017

Academic Editor: Costantino Del Gaudio

Copyright © 2017 Jian Wan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Diabetes mellitus is a disease which has affected 415 million patients in 2015. In an effort to replace the significant demands on transplantation and morbidity associated with transplantation, the production of β-like cells differentiated from induced pluripotent stem cells (iPSCs) was evaluated. This approach is associated with promising decellularized scaffolds with natural extracellular matrix (ECM) and ideal cubic environment that will promote cell growth in vivo. Our efforts focused on combining decellularized rat pancreatic scaffolds with mouse GFP+-iPSCs-derived pancreatic β-like cells, to evaluate whether decellularized scaffolds could facilitate the growth and function of β-like cells. β-like cells were differentiated from GFP+-iPSCs and evaluated via cultivating in the dynamic circulation perfusion device. Our results demonstrated that decellularized pancreatic scaffolds display favorable biochemical properties. Furthermore, not only could the scaffolds support the survival of β-like cells, but they also accelerated the expression of the insulin as compared to plate-based cell culture. In conclusion, these results suggest that decellularized pancreatic scaffolds could provide a suitable platform for cellular activities of β-like cells including survival and insulin secretion. This study provides preliminary support for regenerating insulin-secreting organs from the decellularized scaffolds combined with iPSCs derived β-like cells as a potential clinical application.