Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2017, Article ID 4276928, 14 pages
https://doi.org/10.1155/2017/4276928
Research Article

Culture of iPSCs Derived Pancreatic β-Like Cells In Vitro Using Decellularized Pancreatic Scaffolds: A Preliminary Trial

1Department of General Surgery, Affiliated Hospital of Nantong University, Nan Tong, Jiang Su, China
2Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nan Tong, Jiang Su, China
3Department of General Surgery, Affiliated Cancer Hospital of Nantong University, Nan Tong, Jiang Su, China
4Department of Emergency Surgery, Affiliated Hospital of Nantong University, Nan Tong, Jiang Su, China

Correspondence should be addressed to Yuhua Lu; moc.621@67hyl and Zhiwei Wang; moc.361@9363wzw

Received 31 August 2016; Revised 30 December 2016; Accepted 19 January 2017; Published 5 April 2017

Academic Editor: Costantino Del Gaudio

Copyright © 2017 Jian Wan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. O. Veiseh, B. C. Tang, K. A. Whitehead, D. G. Anderson, and R. Langer, “Managing diabetes with nanomedicine: challenges and opportunities,” Nature Reviews Drug Discovery, vol. 14, no. 1, pp. 45–57, 2014. View at Publisher · View at Google Scholar · View at Scopus
  2. J. C. Pickup, “Insulin-pump therapy for type 1 diabetes mellitus,” The New England Journal of Medicine, vol. 366, no. 17, pp. 1616–1624, 2012. View at Publisher · View at Google Scholar · View at Scopus
  3. W. Truong, J. R. T. Lakey, E. A. Ryan, and A. M. J. Shapiro, “Clinical islet transplantation at the University of Alberta—the Edmonton experience,” Clinical Transplants, pp. 153–172, 2005. View at Google Scholar · View at Scopus
  4. A. J. Ahearn, J. R. Parekh, and A. M. Posselt, “Islet transplantation for Type 1 diabetes: where are we now?” Expert Review of Clinical Immunology, vol. 11, no. 1, pp. 59–68, 2015. View at Publisher · View at Google Scholar · View at Scopus
  5. R. P. Robertson, “Islet transplantation as a treatment for diabetes—a work in progress,” The New England Journal of Medicine, vol. 350, no. 7, pp. 694–705, 2004. View at Publisher · View at Google Scholar · View at Scopus
  6. D. Zhang, W. Jiang, M. Liu et al., “Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells,” Cell Research, vol. 19, no. 4, pp. 429–438, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. A. Rezania, J. E. Bruin, P. Arora et al., “Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells,” Nature Biotechnology, vol. 32, no. 11, pp. 1121–1133, 2014. View at Publisher · View at Google Scholar · View at Scopus
  8. R. F. El-Demerdash, L. N. Hammad, M. M. Kamal, and H. O. El Mesallamy, “A comparison of Wharton's jelly and cord blood as a source of mesenchymal stem cells for diabetes cell therapy,” Regenerative Medicine, vol. 10, no. 7, pp. 841–855, 2015. View at Publisher · View at Google Scholar · View at Scopus
  9. Z. Alipio, W. Liao, E. J. Roemer et al., “Reversal of hyperglycemia in diabetic mouse models using induced-pluripotent stem (iPS)-derived pancreatic β-like cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 30, pp. 13426–13431, 2010. View at Publisher · View at Google Scholar · View at Scopus
  10. B. Nilsson, K. N. Ekdahl, and O. Korsgren, “Control of instant blood-mediated inflammatory reaction to improve islets of Langerhans engraftment,” Current Opinion in Organ Transplantation, vol. 16, no. 6, pp. 620–626, 2011. View at Publisher · View at Google Scholar · View at Scopus
  11. B. Oberwallner, A. Brodarac, Y.-H. Choi et al., “Preparation of cardiac extracellular matrix scaffolds by decellularization of human myocardium,” Journal of Biomedical Materials Research - Part A, vol. 102, no. 9, pp. 3263–3272, 2014. View at Publisher · View at Google Scholar · View at Scopus
  12. T.-Y. Lu, B. Lin, J. Kim et al., “Repopulation of decellularized mouse heart with human induced pluripotent stem cell-derived cardiovascular progenitor cells,” Nature Communications, vol. 4, article no. 2307, 2013. View at Publisher · View at Google Scholar · View at Scopus
  13. M. Ghaedi, E. A. Calle, J. J. Mendez et al., “Human iPS cell-derived alveolar epithelium repopulates lung extracellular matrix,” The Journal of Clinical Investigation, vol. 123, no. 11, pp. 4950–4962, 2013. View at Publisher · View at Google Scholar · View at Scopus
  14. B. E. Uygun, A. Soto-Gutierrez, H. Yagi et al., “Organ reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix,” Nature Medicine, vol. 16, no. 7, pp. 814–820, 2010. View at Publisher · View at Google Scholar · View at Scopus
  15. R. Lang, M. M. Stern, L. Smith et al., “Three-dimensional culture of hepatocytes on porcine liver tissue-derived extracellular matrix,” Biomaterials, vol. 32, no. 29, pp. 7042–7052, 2011. View at Publisher · View at Google Scholar · View at Scopus
  16. J. J. Song, J. P. Guyette, S. E. Gilpin, G. Gonzalez, J. P. Vacanti, and H. C. Ott, “Regeneration and experimental orthotopic transplantation of a bioengineered kidney,” Nature Medicine, vol. 19, no. 5, pp. 646–651, 2013. View at Publisher · View at Google Scholar · View at Scopus
  17. S.-K. Goh, S. Bertera, P. Olsen et al., “Perfusion-decellularized pancreas as a natural 3D scaffold for pancreatic tissue and whole organ engineering,” Biomaterials, vol. 34, no. 28, pp. 6760–6772, 2013. View at Publisher · View at Google Scholar · View at Scopus
  18. P. Zhou, Y. Guo, Y. Huang et al., “The dynamic three-dimensional culture of islet-like clusters in decellularized liver scaffolds,” Cell and Tissue Research, vol. 365, no. 1, pp. 157–171, 2016. View at Publisher · View at Google Scholar · View at Scopus
  19. T. Xu, M. Zhu, Y. Guo et al., “Three-dimensional culture of mouse pancreatic islet on a liver-derived perfusion-decellularized bioscaffold for potential clinical application,” Journal of Biomaterials Applications, vol. 30, no. 4, pp. 379–387, 2015. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Jiang, W. Lv, X. Ye et al., “Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation,” Cell Research, vol. 23, no. 1, pp. 92–106, 2013. View at Publisher · View at Google Scholar · View at Scopus
  21. K. P. Smith, M. X. Luong, and G. S. Stein, “Pluripotency: toward a gold standard for human ES and iPS cells,” Journal of Cellular Physiology, vol. 220, no. 1, pp. 21–29, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. I. S. Schroeder, A. Rolletschek, P. Blyszczuk, G. Kania, and A. M. Wobus, “Differentiation of mouse embryonic stem cells to insulin-producing cells,” Nature Protocols, vol. 1, no. 2, pp. 495–507, 2006. View at Publisher · View at Google Scholar · View at Scopus
  23. T. Kobayashi, T. Yamaguchi, S. Hamanaka et al., “Generation of rat pancreas in mouse by interspecific blastocyst injection of pluripotent stem cells,” Cell, vol. 142, no. 5, pp. 787–799, 2010. View at Publisher · View at Google Scholar · View at Scopus
  24. K. Takahashi and S. Yamanaka, “Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors,” Cell, vol. 126, no. 4, pp. 663–676, 2006. View at Publisher · View at Google Scholar · View at Scopus
  25. A. Rakovic, P. Seibler, and C. Klein, “IPS models of Parkin and PINK1,” Biochemical Society Transactions, vol. 43, pp. 303–307, 2015. View at Publisher · View at Google Scholar · View at Scopus
  26. I. de Lázaro, A. Yilmazer, and K. Kostarelos, “Induced pluripotent stem (iPS) cells: a new source for cell-based therapeutics?” Journal of Controlled Release, vol. 185, no. 1, pp. 37–44, 2014. View at Publisher · View at Google Scholar · View at Scopus
  27. M. T. Lam and M. T. Longaker, “Comparison of several attachment methods for human iPS, embryonic and adipose-derived stem cells for tissue engineering,” Journal of Tissue Engineering and Regenerative Medicine, vol. 6, supplement 3, pp. s80–s86, 2012. View at Publisher · View at Google Scholar · View at Scopus
  28. A. M. Martins, G. Vunjak-Novakovic, and R. L. Reis, “The current status of iPS cells in cardiac research and their potential for tissue engineering and regenerative medicine,” Stem Cell Reviews and Reports, vol. 10, no. 2, pp. 177–190, 2014. View at Publisher · View at Google Scholar · View at Scopus
  29. L. Wang, Y. Huang, Q. Guo et al., “Differentiation of iPSCs into insulin-producing cells via adenoviral transfection of PDX-1, NeuroD1 and MafA,” Diabetes Research and Clinical Practice, vol. 104, no. 3, pp. 383–392, 2014. View at Publisher · View at Google Scholar · View at Scopus
  30. L. Wang, M. Zhu, Q. Guo et al., “Comparing the reprogramming efficiency of mouse embryonic fibroblasts, mouse bone marrow mesenchymal stem cells and bone marrow mononuclear cells to iPSCs,” In Vitro Cellular and Developmental Biology - Animal, vol. 48, no. 4, pp. 236–243, 2012. View at Publisher · View at Google Scholar · View at Scopus
  31. X. Li, Z.-Y. Shan, Y.-S. Wu et al., “Generation of neural progenitors from induced Bama miniature pig pluripotent cells,” Reproduction, vol. 147, no. 1, pp. 65–72, 2014. View at Publisher · View at Google Scholar · View at Scopus
  32. D. K. Singla, X. Long, C. Glass, R. D. Singla, and B. Yan, “Induced pluripotent stem (iPS) cells repair and regenerate infarcted myocardium,” Molecular Pharmaceutics, vol. 8, no. 5, pp. 1573–1581, 2011. View at Publisher · View at Google Scholar · View at Scopus
  33. G. Amabile, R. S. Welner, C. Nombela-Arrieta et al., “In vivo generation of transplantable human hematopoietic cells from induced pluripotent stem cells,” Blood, vol. 121, no. 8, pp. 1255–1264, 2013. View at Publisher · View at Google Scholar · View at Scopus
  34. A. Soejitno and P. K. A. Prayudi, “The prospect of induced pluripotent stem cells for diabetes mellitus treatment,” Therapeutic Advances in Endocrinology and Metabolism, vol. 2, no. 5, pp. 197–210, 2011. View at Publisher · View at Google Scholar · View at Scopus
  35. H. Kaneto, T. Miyatsuka, T. Shiraiwa et al., “Crucial role of PDX-1 in pancreas development, β-cell differentiation, and induction of surrogate β-cells,” Current Medicinal Chemistry, vol. 14, no. 16, pp. 1745–1752, 2007. View at Publisher · View at Google Scholar · View at Scopus
  36. B. N. Ediger, A. Du, J. Liu et al., “Islet-1 is essential for pancreatic β-cell function,” Diabetes, vol. 63, no. 12, pp. 4206–4217, 2014. View at Publisher · View at Google Scholar · View at Scopus
  37. B. Taylor, F.-F. Liu, and M. Sander, “Nkx6.1 is essential for maintaining the functional state of pancreatic beta cells,” Cell Reports, vol. 4, no. 6, pp. 1262–1275, 2013. View at Publisher · View at Google Scholar · View at Scopus
  38. L. Wilding and M. Gannon, “The role of pdx1 and HNF6 in proliferation and differentiation of endocrine precursors,” Diabetes/Metabolism Research and Reviews, vol. 20, no. 2, pp. 114–123, 2004. View at Publisher · View at Google Scholar · View at Scopus
  39. R. C. Sartore, P. B. Campos, C. A. Trujillo et al., “Retinoic acid-treated pluripotent stem cells undergoing neurogenesis present increased aneuploidy and micronuclei formation,” PLoS ONE, vol. 6, no. 6, Article ID e20667, 2011. View at Publisher · View at Google Scholar · View at Scopus
  40. V. R. Haas, A. R. Santos Jr., and M. L. F. Wada, “Behaviour of fibroblastic cells cultured in collagen I using the sandwich technique,” Cytobios, vol. 413, supplement 2, pp. 255–267, 2001. View at Google Scholar · View at Scopus
  41. P. M. Baptista, M. M. Siddiqui, G. Lozier, S. R. Rodriguez, A. Atala, and S. Soker, “The use of whole organ decellularization for the generation of a vascularized liver organoid,” Hepatology, vol. 53, no. 2, pp. 604–617, 2011. View at Publisher · View at Google Scholar · View at Scopus
  42. T. J. Keane, R. Londono, N. J. Turner, and S. F. Badylak, “Consequences of ineffective decellularization of biologic scaffolds on the host response,” Biomaterials, vol. 33, no. 6, pp. 1771–1781, 2012. View at Publisher · View at Google Scholar · View at Scopus
  43. A. B. Daly, J. M. Wallis, Z. D. Borg et al., “Initial binding and recellularization of decellularized mouse lung scaffolds with bone marrow-derived mesenchymal stromal cells,” Tissue Engineering Part A, vol. 18, no. 1-2, pp. 1–16, 2012. View at Publisher · View at Google Scholar · View at Scopus
  44. J. E. Frith, R. J. Mills, J. E. Hudson, and J. J. Cooper-White, “Tailored integrin-extracellular matrix interactions to direct human mesenchymal stem cell differentiation,” Stem Cells and Development, vol. 21, no. 13, pp. 2442–2456, 2012. View at Publisher · View at Google Scholar · View at Scopus
  45. P. Zhou, Y. Huang, Y. Guo et al., “Decellularization and recellularization of rat livers with hepatocytes and endothelial progenitor cells,” Artificial Organs, vol. 40, no. 3, pp. E25–E38, 2016. View at Publisher · View at Google Scholar · View at Scopus