Culture of iPSCs Derived Pancreatic β-Like Cells In Vitro Using Decellularized Pancreatic Scaffolds: A Preliminary Trial

Table 1

PCR primer sequences for specific pancreatic markers.

Gene	GenBank accession number	Primer
GAPDH	NM_000072.6
Forward primer		AAGAAGGTGGTGAAGCAGG
Reverse primer		GAAGGTGGAAGAGTGGGAGT
Insulin	NM_000085.6
Forward primer		AGTTGAGTTGGGCAGAATAGG
Reverse primer		TCCAAAGGGCACCGTAT
Glucagon	NM_000068.7
Forward primer		CCAGCGACTACAGCAAATACC
Reverse primer		GAGAAGGAGCCATCAGCGT
HNF6	NM_000075.6
Forward primer		CGTTACAGCATCCCACAG
Reverse primer		AGCCACTTCCACATCCTC
Nkx6.1	NM_144955.2
Forward primer		GAAAACACACCAGACCCA
Reverse primer		GGAACCAGACCTTGACCT
Islet-1	NM_021459.4
Forward primer		CACCTTGCGGACCTGCTAT
Reverse primer		AGGGCGGCTGGTAACTTTG
Pdx-1	NM_008814.3
Forward primer		CGGAACCCGAGGAAAACA
Reverse primer		CGAGGTCACCGCACAATCT