Basic Fibroblast Growth Factor-Anchored Multilayered Mesenchymal Cell Sheets Accelerate Periosteal Bone Formation

Figure 1

Construction of multilayered tissues using a cell-accumulation technique with fibronectin-gelatin (FN-G) nanofilms. (a) Analysis of cell-surface markers in isolated periosteal mesenchymal cells. Blue: nonstaining control. Red: stained sample. (b) Hematoxylin-eosin (HE) staining of the multilayered mesenchymal cells. (b1) non-FN-G-coated cells and (b2) FN-G-coated cells. Scale bar = 50 μm.

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