MicroRNA Mediating Networks in Granulosa Cells Associated with Ovarian Follicular Development

Figure 8

The effect of miR-26a/b on the regulating smad2 mRNA in KGN cells. (a) A sketch map describes predicted seed sequences of miR-26a/b in the conservative 3′-UTR of smad2 in several mammal species. (b) The transfection efficiency of overexpression vectors of miR-26a/b (pSUPER-miR-26a and pSUPER-miR-26b) was validated by RT PCR. (c, d, and e) The effects of pSUPER-miR-26a/b, miR-26a/b mimics, and miR-26a/b inhibitors on the smad2 mRNA level were identified in KGN cells, respectively. , , and ; all experiments were independently repeated three times.

(a)

(b)

(c)

(d)

(e)