Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium

Figure 2

Effect of serum on culture-induced changes in PR isoform expression in vitro: relative mRNA abundance of PR-T, PR-A, and PR-B was measured in term nonlaboring myometrial tissue samples following 48 h incubation in serum-free media or media supplemented with 5% CSS () and expressed relative to Alien reference. In addition, PR-A/PR-B expression ratio was calculated. (a) PR-T mRNA abundance. (b) PR-A mRNA abundance. (c) PR-B mRNA abundance. (d) PR-A/PR-B expression ratio. Data was checked for normality (Shapiro-Wilk normality test) and if necessary was logarithmically transformed to approach normal distribution (Shapiro-Wilk normality test). Data was analysed using 1-way ANOVA with multiple comparisons (Dunnett). Data are mean ± SEM.

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