Jacalin Has Chemopreventive Effects on Colon Cancer Development

Figure 3

Jacalin treatment affects colonic epithelial cell proliferation but not apoptosis. (a) Proliferative activity in the colon was assessed by using proliferating cell nuclear antigen (PCNA) antibody. The index of colonic crypt cells expressing proliferating cell nuclear antigen (PCNA-i) was calculated from the ratio of positively stained nuclei to a total number of nuclei counted. (b) Apoptotic cells were detected by caspase-3 immunoreactivity in colonic stromal cells. Apoptosis index shows the number of stained cells in the intercrypt spaces. (c) Representative immunohistochemistry images of proliferating cells of the control group; (d) MNNG-exposed group; (e) MNNG-exposed group treated with jacalin 0.5 μg; and (f) MNNG-exposed group treated with jacalin 25 μg. Results are expressed as mean ± SEM for each group. Statistical analysis: (a) one-way analysis of variance and (b) one-way ANOVA (Kruskal-Wallis) and Dunn’s multiple comparison post hoc tests. to control group. to PBS group. ; or .

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