Figure 2: L. donovani PF16 null mutants are immotile. LdB pTB007 or LdB pTB007 ΔPF16 promastigotes in logarithmic phase were filmed for 10 s (200 frames). The tracking of individual parasites was performed using the Spot Detector and Spot Tracking tools from Icy software. Images were taken with a Leica DMI 4000B microscope, using a 40x objective and an Evolv EMCCD camera, with Metaview software.