Santamarine Inhibits NF-κB Activation and Induces Mitochondrial Apoptosis in A549 Lung Adenocarcinoma Cells via Oxidative Stress

Figure 5

STM inhibits phosphorylation of IκB-α in A549 cells. (a) Cells were treated with STM as indicated for 24 h. Total cell lysates were extracted and subjected to western blot for the expression and phosphorylation of IκB-α. GAPDH was used as a loading control. (b) Cells were incubated with or without 60 μM STM for 1 h and then stimulated with TNF-α for 1 h. Total cell lysates were extracted and subjected to western blot for the expression and phosphorylation of IκB-α.

(a)

(b)