Curcumin Analogue CA15 Exhibits Anticancer Effects on HEp-2 Cells via Targeting NF-κB

Figure 4

CA15 inhibited TNF-α-induced NF-κB activation in HEp-2 cells. (a and b) HEp-2 cells were pretreated with different concentrations (0–20 μM) of CA15 and 20 μM BMS for 1 h followed by incubation with TNF-α (5 ng/ml) for 15 min (a) or 30 min (b). The protein levels of p-IKK, IKK, and IκB were examined by Western blot. The graphs display means ± SEM of 3 independent experiments. < 0.01 and < 0.001 versus TNF-α treated group. (c) HEp-2 cells were pretreated with 20 μM CA15 and 20 μM BMS for 1 h followed by incubation with TNF-α (5 ng/ml) for 60 min. Cells were then incubated with NF-κB-p65 antibody and PE-conjugated secondary antibody (red), and the nuclei were stained with DAPI (blue). The photographs were obtained by fluorescence microscope (magnification ×200). TNF-α: tumor necrosis factor-α; Cur: curcumin; BMS: BMS-345541.

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