Research Article

Specific-Locus Amplified Fragment Sequencing Reveals Spontaneous Single-Nucleotide Mutations in Rice OsMsh6 Mutants

Table 1

SLAFs and sequencing data used in this research.

Mutant linesSLAF numbersTotal depthAverage depthTotal readsQ30 (%)GC%

ND6011/G410,743115,81010.78180,76087.8844.77
ND6011/G510,58591,6668.66151,38888.2244.76
ND6011/G610,572112,38010.63175,84687.7045.34
ND6011/G710,806157,01114.53250,09886.6844.9
NF7784/G410,760124,17011.54186,34287.0845.15
NF7784/G510,685100,8669.44163,09686.6944.45
NF7784/G610,812144,66513.38242,70787.1444.84
NF7784/G710,696114,23310.68241,55886.0244.85
NF9010/G410,767138,78712.89248,65287.1544.41
NF9010/G510,799165,22515.3249,92286.7145.41
NF9010/G610,813172,57515.96274,44487.6045.08
NF9010/G710,798157,86714.62254,22487.0344.26
Msh6WT10,688177,20716.58266,00688.7245.27