Specific-Locus Amplified Fragment Sequencing Reveals Spontaneous Single-Nucleotide Mutations in Rice OsMsh6 Mutants

Table 5

Ratios of observed/expected frequencies on either side of the mutated base.

Flanking base				Mutated base	+1	+2	+3
A	1.10	1.01	0.85	A	0.93	1.01	0.99
C	0.97	1.13	1.51	A	1.03	1.01	0.78
G	0.81	0.72	0.62	A	0.94	0.75	0.96
T	1.12	1.13	1.01	A	1.10	1.22	1.28
	4.21	7.71	29.44		1.38	7.59	8.76
	0.240	0.053			0.710	0.055	0.033
A	1.10	0.77	1.20	C	0.98	1.09	1.12
C	1.02	0.88	0.90	C	0.83	0.64	0.96
G	0.80	1.09	0.86	C	1.36	1.25	0.88
T	1.07	1.26	1.04	C	0.83	1.02	1.04
	3.54	9.10	4.43		11.66	12.46	2.00
	0.316	0.028	0.218		0.009	0.006	0.572
A	0.94	0.88	1.02	G	1.06	1.25	1.19
C	0.94	1.33	1.33	G	0.92	0.77	0.83
G	0.79	0.73	0.71	G	1.02	0.88	0.96
T	1.33	1.06	0.94	G	1.00	1.10	1.02
	8.23	10.19	10.08		0.50	7.19	3.58
	0.041	0.017	0.018		0.919	0.066	0.311
A	1.12	1.05	1.12	T	1.09	1.15	1.15
C	0.91	0.76	1.14	T	0.61	0.79	0.79
G	0.98	1.12	1.08	T	1.50	1.15	1.15
T	0.98	1.08	0.67	T	0.80	0.91	0.91
	1.55	5.36	9.91		29.85	6.55	6.55
	0.672	0.147	0.019			0.088	0.088