Production of Human Cu,Zn SOD with Higher Activity and Lower Toxicity in<i> E. coli</i> via Mutation of Free Cysteine Residues

<div>Construction and expression levels of wtSOD1 and its mutants in<i> E. coli</i>. (a) Schematic diagram of the molecular structure of the wild-type and mutant SODs constructed. Blue color indicates the wild-type DNA or amino acid sequences and red color indicates the mutant ones. (b) Expression of wtSOD1 and its mutants by SDS-PAGE analysis. Lane MW: molecular weight standard ladder (kDa). Lane 1: whole cell lysate of wtSOD1-expressing cells before induction. Lanes 2–5: whole cell lysate of cells expressing wtSOD1 (lane 2), mhSOD1/C6S (lane 3), mhSOD1/C111S (lane 4), and mhSOD1/C6S/C111S (lane 5) after induction.</div>

BioMed Research International

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Figure 1

Figure 1: Production of Human Cu,Zn SOD with Higher Activity and Lower Toxicity in<i> E. coli</i> via Mutation of Free Cysteine Residues 

BioMed Research International

Production of Human Cu,Zn SOD with Higher Activity and Lower Toxicity in E. coli via Mutation of Free Cysteine Residues

Figure 1