Production of Human Cu,Zn SOD with Higher Activity and Lower Toxicity in E. coli via Mutation of Free Cysteine Residues

Figure 6

Whitening effects of wtSOD1 and mhSOD1/C111S. (a) B16 cells were treated with both wtSOD1 and mhSOD1/C111S diluted with RPMI 1640 medium and cell proliferation was analyzed by MTT assay. ((b) and (c)) The inhibition of mushroom tyrosinase activity by wtSOD1 and mhSOD1/C111S was measured in vitro (b) and in vivo (c). compared between indicated groups.

(a)

(b)

(c)