Production of Human Cu,Zn SOD with Higher Activity and Lower Toxicity in E. coli via Mutation of Free Cysteine Residues

Figure 7

Antiradiation effects of SOD1s. (a) BHK cells were treated with wtSOD1 and mhSOD1/C111S and cell proliferation was analyzed by MTT assay. Kojic acid and arbutin were used as positive controls. (b) BHK cells were treated with serially diluted wtSOD1 and mhSOD1/C111S and cell proliferation was analyzed by MTT assay. ; ; compared between indicated groups. (c) BHK cells were treated with wtSOD1 and mhSOD1/C111S for 3 h, followed by UV radiation for 20 min, and DNA damage was analyzed by agarose gel electrophoresis. Data are representative of three experiments.

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