Computational Identification and Characterization of a Promiscuous T-Cell Epitope on the Extracellular Protein 85B of Mycobacterium spp. for Peptide-Based Subunit Vaccine Design

Table 4

Binding of 181-QQFIYAGSLSALLDP-195 epitope of Ag85B to respective allele predicted by NetMHCII 2.2 servers.

MHC II alleles	Promiscuous peptide sequence		Affinity (nM)
	Full peptide (15 mer)	Core peptide (9 mer)
HLA-DRB10101	QQFIYAGSLSALLDP	FIYAGSLSA	4.5
HLA-DRB10404	QQFIYAGSLSALLDP	FIYAGSLSA	49.1
HLA-DRB10405	QQFIYAGSLSALLDP	FIYAGSLSA	20.7
HLA-DRB10701	QQFIYAGSLSALLDP	FIYAGSLSA	6.1
HLA-DRB10901	QQFIYAGSLSALLDP	FIYAGSLSA	9.2
HLA-DRB50101	QQFIYAGSLSALLDP	FIYAGSLSA	33.7
HLA-DPA10201-DPB10101	QQFIYAGSLSALLDP	FIYAGSLSA	38.1
HLA-DQA10501-DQB10301	QQFIYAGSLSALLDP	FIYAGSLSA	5.7

Binding affinity below 50 nM is considered as strong immunogenicity.