Computational Identification and Characterization of a Promiscuous T-Cell Epitope on the Extracellular Protein 85B of Mycobacterium spp. for Peptide-Based Subunit Vaccine Design

Table 5

Binding of epitopes of Ag85B from M. tuberculosis against HLA-DRB10701 molecules.

Epitope rank	Start position	Sequence	Score
Predicted with ProPed
1	271	FVRSSNLKF	8
2	166	MAGSSAMIL	5.9
3	154	VKPTGSAAI	5.8
4	184	YAGSLSALL	5.7
5	101	YQSGLSIVM	5.3
Predicted with SYFPEITHI
1	152	NRAVKPTGSAAIGLS	32
2	182	QFIYAGSLSALLDS	32
3	162	AIGLSMAGSSAMILA	30
4	180	PQQFIYAGSLSALLD	28
5	217	ADMWGPSSDPAWERN	28

Binding of epitopes of Ag85B from Mycobacterium spp. was predicted by analyzing with ProPred and SYFPEITHI servers. The higher the score of an epitope, the greater the probability of binding to a given MHC molecule. The core sequence (9-mer) of the epitope 181-QQFIYAGSLSALLDP-195 is italicized. Five epitopes with the top scores are shown.