Peptide Fraction pOh2 Exerts Antiadipogenic Activity through Inhibition of C/EBP-α and PPAR-γ Expression in 3T3-L1 Adipocytes

Figure 4

Effect of pOh1 and pOh2 on adipogenesis in 3T3-L1 adipocytes. (a) Intracellular lipid was stained with Oil Red O on differentiated 3T3-L1 adipocytes incubated only with an adipogenesis cocktail (i) or with an adipogenesis cocktail and supplemented with 1 μg/mL of pOh1 (ii), 10 μg/mL of pOh1 (iii), 1 μg/mL of pOh2 (iv), and 10 μg/mL of pOh2 (v). (b) Optical density of extracted dye was monitored at 510 nm. (c) The viability of 3T3-L1 adipocytes. Results shown are the means of three independent experiments conducted in triplicate. NS, not significant; and as compared with differentiated 3T3-L1 adipocytes.

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