Rapid Identification and Isolation of Inhibitors of Rat Lens Aldose Reductase and Antioxidant in Maackia amurensis

Figure 3

HSCCC separation of active compounds from an ethyl acetate fraction of Maackia amurensis. (a) The upper layer from an ethyl acetate fraction of Maackia amurensis from an HSCCC separation solvent system: n-hexane-EtOAc-methanol-water (2.5 : 7.5 : 5 : 5, v/v); flow rate, 4.0 mL/min; revolution speed, 500 rpm; sample size, 2.0 g; injection volume, 40 mL; detection wavelength, 254 nm. (b) HPLC analysis of isolated compounds by an HSCCC system; peak 5, piceatannol; peak 6, resveratrol; peak 7, daidzein; peak 8, genistein; peak 9, formononetin.

(a)

(b)