Rapid Identification and Isolation of Inhibitors of Rat Lens Aldose Reductase and Antioxidant in Maackia amurensis

Table 1

Effects of Maackia amurensis fractions in terms of antioxidation and inhibition of aldose reductase in rat lens.

Test sample	Antioxidant		RLAR inhibition
	IC50 (μg/mL)	TEAC	IC50 (μg/mL)
Crude	12.03 ± 0.02	0.72	35.6 ± 3.82
n-Hex fraction	134.02 ± 0.78	0.07	>100
CH2Cl2 fraction	141.13 ± 0.78	0.06	>100
EtOAc fraction	4.92 ± 0.02	1.77	11.3 ± 4.31
n-BuOH fraction	96.48 ± 0.45	0.09	>100
H2O fraction	282.62 ± 0.38	0.03	>100
Trolox	8.72 ± 0.01	1.00	—
Quercetin	—	—	3.30 ± 3.20

The IC₅₀ value was defined as the concentration of the 50% inhibition.
Trolox equivalent antioxidant capacity.
Trolox was used as positive control for ABTS assay.
Quercetin was used as positive control for RLAR inhibitory activity measurement.