Research Article

Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes

Table 1

Ingredients, chemical composition, and fatty acids profile of the experimental concentrates and of the hay and rolled barley administered to the ewes.

Grass hayRolled barleyControl dietCHT dietQUE diet

Ingredients (g kg of DM)
 Barley213.8213.8213.8
 Corn211.3211.3211.3
 Wheat bran158.5158.5158.5
 Soybean meal (44 CP)126.8126.8126.8
 Beet pulp89.889.889.8
 Soybean oil184.584.584.5
 Bentonite52.8--
 Chestnut tannin extract2-52.8-
 Quebracho tannin extract3--52.8
 Molasses41.341.341.3
 CaCO310.610.610.6
 Sodium bicarbonate5.35.35.3
 Dicalcium phosphate5.35.35.3
Chemical composition (g kgof DM)
 Organic matter847.0859.9816.9858.1869.6
 Crude protein111.2121.0165.6173.7170.3
 Ether extract12.016.1109.4105.4102.4
 NDF636.4134.1174.7181.4172.1
 ADF501.354.277.672.474.3
 ADL105.714.910.613.38.7
 Ash69.621.084.639.939.4
 ME (MJ kg−1 DM)7.89.913.114.114.1
 NEl (Mcal kg−1 DM)0.91.22.02.12.1
Fatty acids (g/100 g of total fatty acids)
 16:035.518.214.014.414.9
 SA, 18:05.84.63.63.43.4
 18:1 cis99.321.223.322.922.0
 LA, 18:2 cis9 cis1228.545.051.451.751.8
 LNA, 18:3 cis9 cis12 cis152.86.05.85.65.8
 Other FA18.14.91.92.02.1

Fatty acid profile of soybean oil (g/100 g of total fatty acids): C16:0, 11.01; C18:0, 3.6; C18:1 cis9, 22.09; C18:2 cis9 and cis12, 53.7; C18:3 cis9, cis12, and cis15, 7.2. Hydrolysable tannins extracted from chestnut wood (Castanea sativa Mill.) containing 750 g of equivalent tannic acid/kg DM (provided by Gruppo Mauro Saviola Srl Radicofani, Siena, Italy). Condensed tannins extracted from quebracho (Schinopsis lorentzii) containing 456 g of equivalent tannic acid/kg DM (provided by Guido Lapi SpA, Castel Franco di Sotto, Pisa, Italy).