Obesity Induces Artery-Specific Alterations: Evaluation of Vascular Function and Inflammatory and Smooth Muscle Phenotypic Markers

Table 1

Primers used in real-time PCR analysis.

Gene	Gene name	Sequence
THRIL	TNF-	F – ATGAGCACAGAAAGCATGATC
		R – TACAGGCTTGTCACTCGAATT
IL1B	IL-1β	F – TGACCCATGTGAGCTGAAAG
		R – GGGATTTTGTCGTTGCTTGT
IL6	IL-6	F – ACCACCCACAACAGACCAGT
		R – CAGAATTGCCATTGCACAAC
IL10	IL-10	F – CATGGGTCTTGGGAAGAGAA
		R – GCTTTCGAGACTGGAAGTGG
PTGS2	COX-2	F – AGATCAGAAGCGAGGACCTG
		R – CCATCCTGGAAAAGTCGAAG
NOS2	iNOS	F – GGATATCTTCGGTGCGGTCTT
		R – GCTGTAACTCTTCTGGGTGTCAGA
COL3A1	Type III collagen	F – GGGATCCAATGAGGGAGAAT
		R – GGCCTTGCGTGTTTGATATT
NOTCH3	Notch3	F – GCGTTTGCCAGAGTTCAGTG
		R – GAGCAGTCTGGGCCTTGGAA
TAGLN	SM22	F – TGCGTGGCTACATTGGGAAT
		R – AATGCTTGGAGCAAGGGTCA
ACTA2	SM -actin	F – CCATCTTTCATTGGGATGGAGTCA
		R – AATGCCTGGGTACATGGTGGTA
ACTN1	-Actinin	F – CCACTTTGACCGGGATCACT
		R – CGGTTGGGGTCTACAATGCT
CALD1	Caldesmon	F – GCCACACTCTCTCAAATTGCC
		R – CACCTGGTCTGTCACCTGTC
MYLK	MLCK	F – CCAGTTACCTGGGAAGACCAT
		R – ACCTTCGAGGGATCCACACT
TPM2	Tropomyosin	F – TATGAGGAGGTGGCCAGGAA
		R – TGGTCCATGGTTCGAAGCTC
PPIA	Cyclophilin	F – TATCTGCACTGCCAAGACTGAAT
		R – CTTCTTGCTGGTCTTGCCATTCC

TNF, tumor necrosis factor; IL, interleukin; COX, cyclooxygenase; iNOS, inducible nitric oxide synthase; SM, smooth muscle; MLCK, myosin light chain kinase; F, forward; R, reverse.